Human CD200 | HUABIO HA722380 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CD200

catalog :

HA722380

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-60

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human CD200 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722379) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted recombinant human CD200 protein (HA210665) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA722380, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native CD200 in human serum samples. The concentrations of CD200 were interpolated from the CD200 standard curves and corrected for sample dilution. Undiluted samples are human serum 100%. The mean CD200 concentration was determined to be 652.9 pg/ml in human serum.

image 3 :

Interpolated concentrations of native CD200 in SK-Mel-28 and NCI-H226 extract samples based on a 1,000 µg/ml extract load. The concentrations of CD200 were measured in triplicates, interpolated from the CD200 standard curve and corrected for sample dilution. Undiluted samples are SK-Mel-28 extract 20% and NCI-H226 extract 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean CD200 concentration was determined to be 3,860 pg/ml in SK-Mel-28 extract and 21,265 pg/ml in NCI-H226 extract.

product information

SKU :

HA722380

Target name :

Human CD200

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD200 aa 31-232 (Extracellular).

Uniprot id :

P41217>SwissProt: P41217 Human

Host :

Rabbit

Clone number :

PSH05-60

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant human CD200 protein (HA210665).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-59] to Human CD200 (Capture) (HA722379) and recombinant standard Human CD200 protein (HA210665). The reference range value is 30.9-2500pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722380_4.png

Pic legend4 :

Interpolated concentrations of spiked CD200 in human cell culture media samples. The concentrations of CD200 were measured in triplicates, interpolated from the CD200 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

more info or order :

HUABIO product webpage