product summary
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company name :
HUABIO
product type :
antibody
product name :
ENDOGL1 / ENGL
catalog :
HA722356
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE63-12
reactivity :
human
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of ENDOGL1 / ENGL on different lysates with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722356) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
ENDOGL1 / ENGL was immunoprecipitated from 0.2 mg HUVEC cell lysate with HA722356 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722356 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HUVEC cell lysate (input)
Lane 2: HA722356 IP in HUVEC cell lysate
Lane 3: Rabbit IgG instead of HA722356 in HUVEC cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
product information
SKU :
HA722356
Target name :
ENDOGL1 / ENGL
Species reactivity :
Human
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ENDOGL1 / ENGL aa 341-390 / 368.
Uniprot id :
Q9Y2C4>SwissProt: Q9Y2C4 Human
Host :
Rabbit
Clone number :
JE63-12
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HepG2 cell lysate, HUVEC cell lysate, HL-60 cell lysate, MCF7 cell lysate, human heart tissue.
Molecular wt :
Predicted band size: 41 kDa
Subcellular location :
Mitochondrion inner membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200-1:1,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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