product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
NELF-B
catalog :
HA722320
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE77-56
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
HUABIO HA722320 image 1
Western blot analysis of NELF-B on different lysates with Rabbit anti-NELF-B antibody (HA722320) at 1/1,000 dilution. Lane 1: T-47D cell lysate Lane 2: HeLa cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: C2C12 cell lysate Lane 10: C6 cell lysate Lane 11: L6 cell lysate Lane 12: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: Lane 1-7: 2 minutes; Lane 8-12: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722320) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA722320
Target name :
NELF-B
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human NELF-B aa 1-250 / 580.
Uniprot id :
Q8WX92>SwissProt: Q8WX92 Human;SwissProt: Q8C4Y3 Mouse;Entrez Gene: 311796 Rat
Host :
Rabbit
Clone number :
JE77-56
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
T-47D cell lysate, HeLa cell lysate, BxPC-3 cell lysate, HepG2 cell lysate, MCF7 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate.
Molecular wt :
Predicted band size: 66 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
more info or order :
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!