product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-Beta Catenin (T41/S45)
catalog :
HA722316
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE54-02
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Phospho-Beta Catenin (T41/S45) on different lysates with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 5: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, then the membrane treated with λpp for 1 hour
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lane 7: C6 cell lysate
Lane 8: C6 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 9: C6 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 30 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5-9: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722316) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722316
Target name :
Phospho-Beta Catenin (T41/S45)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding Thr41/Ser45 of human Beta catenin.
Uniprot id :
P35222>SwissProt: P35222 Human;SwissProt: Q02248 Mouse;SwissProt: Q9WU82 Rat
Host :
Rabbit
Clone number :
JE54-02
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Molecular wt :
Predicted band size: 85 kDa
Subcellular location :
Cytoplasm, Nucleus, Cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722316_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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