product summary
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company name :
HUABIO
product type :
antibody
product name :
Monoamine Oxidase A / MAO-A
catalog :
HA722313
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE39-96
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Monoamine Oxidase A / MAO-A on different lysates with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (40 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)
Lane 7: Rat small intestine tissue lysate (40 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722313) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722313) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722313) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722313
Target name :
Monoamine Oxidase A / MAO-A
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human MAO-A aa 450-527.
Uniprot id :
P21397>SwissProt: P21397 Human;SwissProt: Q64133 Mouse;SwissProt: P21396 Rat
Host :
Rabbit
Clone number :
JE39-96
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 cell lysate, HUVEC cell lysate, A431 cell lysate, mouse small intestine tissue lysate, mouse kidney tissue lysate, rat small intestine tissue lysate, human kidney tissue, human colon tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.
Molecular wt :
Predicted band size: 60 kDa
Subcellular location :
Mitochondrion outer membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:3,000-1:10,000
;IF-Tissue: 1:200-1:1,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722313_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722313) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722313_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722313) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA722313_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Monoamine Oxidase A / MAO-A antibody (HA722313) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722313) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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