Human CD33 | HUABIO HA722254 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CD33

catalog :

HA722254

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-25

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human CD33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722254) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD33 protein (HA210949) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA722255, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

The concentrations of CD33 were interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 5%. The mean CD33 concentration was determined to be 7,466 pg/mL in mixed serum from twenty-one volunteers.

image 3 :

Interpolated concentrations of native CD33 in U937 and HL-60 extract samples based on a 1,000 µg/mL extract load. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 5% and HL-60 extract 1%. The mean CD33 concentration was determined to be 5,333 pg/mL in U937 extract and 21,225 pg/mL in HL-60 extract.

product information

SKU :

HA722254

Target name :

Human CD33

Species reactivity :

Human

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD33 aa 18-259 (P20138).

Uniprot id :

P20138>SwissProt: P20138 Human

Host :

Rabbit

Clone number :

PSH05-25

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human CD33 protein (HA210949).

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cell membrane; Peroxisome.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-26] to Human CD33 (Detector) (HA722255) and Recombinant Human CD33 protein (HA210949) as the standard. The reference range value is 22.6-5500pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722254_4.png

Pic legend4 :

Interpolated concentrations of spiked CD33 in human cell culture media samples. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage