CD169 | HUABIO HA722246 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD169

catalog :

HA722246

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-21

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD169 on different lysates with Rabbit anti-CD169 antibody (HA722246) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 183 kDa Observed band size: 220 kDa Exposure time: 28 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of THP-1 cells (left) and THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours (right) labeling CD169. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of RAW264.7 cells (left) and RAW264.7 cells treated with 1μg/mL LPS for 24 hours (right) labeling CD169. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722246

Target name :

CD169

Species reactivity :

Human,Mouse

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD169 aa 1-1,000 / 1,709.

Uniprot id :

Q9BZZ2>SwissProt: Q9BZZ2 Human;SwissProt: Q62230 Mouse

Host :

Rabbit

Clone number :

PSH05-21

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, RAW264.7 cell lysate, THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours, RAW264.7 cells treated with 1μg/mL LPS for 24 hours.

Molecular wt :

Predicted band size: 183 kDa

Subcellular location :

Cell membrane, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:2,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage