Presenilin 2 / AD5 | HUABIO HA722243 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Presenilin 2 / AD5

catalog :

HA722243

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-18

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA722243) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: RAW264.7 cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: C2C12 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: C6 cell lysate (20 µg/Lane) Lane 12: Human brain tissue lysate (40 µg/Lane) Lane 13: Mouse brain tissue lysate (40 µg/Lane) Lane 14: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 23 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722243) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722243 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722243 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722243 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722243 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801

product information

SKU :

HA722243

Target name :

Presenilin 2 / AD5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Presenilin 2 aa 300-400.

Uniprot id :

P49810>SwissProt: P49810 Human;SwissProt: Q61144 Mouse;SwissProt: O88777 Rat

Host :

Rabbit

Clone number :

PSH05-18

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, 293T cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat liver tissue lysate.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Endoplasmic reticulum membrane, Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage