Phospho-TAK1 (S439) | HUABIO HA722242 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-TAK1 (S439)

catalog :

HA722242

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE44-93

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/25,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722242

Target name :

Phospho-TAK1 (S439)

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser439 of mouse TAK1.

Uniprot id :

O43318>SwissProt: O43318 Human;SwissProt: Q62073 Mouse;SwissProt: P0C8E4 Rat

Host :

Rabbit

Clone number :

JE44-93

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, K-562 cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.

Molecular wt :

Predicted band size: 67 kDa

Subcellular location :

Cytoplasm, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:25,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage