Caspase-8 | HUABIO HA722181 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Caspase-8

catalog :

HA722181

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-77

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (HA722181) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (30 µg/Lane) Lane 2: Jurkat treated with 25μM Etoposide for 5 hours cell lysate (30 µg/Lane) Lane 3: Jurkat treated with 25μM Etoposide for 16 hours cell lysate (30 µg/Lane) Lane 4: Jurkat treated with 1μM staurosporine for 3 hours cell lysate (30 µg/Lane) Lane 5: HeLa cell lysate (30 µg/Lane) Lane 6: HeLa treated with 1μM staurosporine for 3 hours cell lysate (30 µg/Lane) Lane 7: HeLa treated with 100μM Etoposide for 4 hours cell lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 18-55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722181) at 1/2,000 dilution was used in antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours labeling Caspase-8 with Rabbit anti-Caspase-8 antibody (HA722181) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-8 antibody (HA722181) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (HA722181) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Caspase-8 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 54,55 kDa Exposure time: 180 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722181) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722181

Target name :

Caspase-8

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Caspase-8 aa 325-374 / 479.

Uniprot id :

Q14790>SwissProt: Q14790 Human

Host :

Rabbit

Clone number :

PSH04-77

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, Jurkat treated with 25μM Etoposide for 5 hours cell lysate, Jurkat treated with 25μM Etoposide for 16 hours cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, HeLa cell lysate, HeLa treated with 1μM staurosporine for 3 hours cell lysate, HeLa treated with 100μM Etoposide for 4 hours cell lysate, human tonsil tissue, human liver tissue, HeLa cells treated with 1μM staurosporine for 3 hours.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722181_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Caspase-8 antibody (HA722181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722181_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caspase-8 antibody (HA722181) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722181) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722181_6.jpg

Pic legend6 :

Flow cytometric analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours cells labeling Caspase-8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722181, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722181_7.jpg

Pic legend7 :

Caspase-8 was immunoprecipitated from 0.2 mg HeLa treated with 1μM staurosporine for 3 hours cell lysate with HA722181 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722181 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 1μM staurosporine for 3 hours cell lysate (input) Lane 2: HA722181 IP in HeLa treated with 1μM staurosporine for 3 hours cell lysate Lane 3: Rabbit IgG instead of HA722181 in HeLa treated with 1μM staurosporine for 3 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 28 seconds; ECL: K1801

more info or order :

HUABIO product webpage