Phospho-Rad17 (S656) | HUABIO HA722148 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Rad17 (S656)

catalog :

HA722148

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE63-30

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Rad17 (S656) on different lysates with Rabbit anti-Phospho-Rad17 (S656) antibody (HA722148) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lane 4: NIH/3T3 cell lysate Lane 5: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate Lane 6: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 43 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722148) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Phospho-Rad17 (S656) with Rabbit anti-Phospho-Rad17 (S656) antibody (HA722148) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Rad17 (S656) antibody (HA722148) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Phospho-Rad17 (S656). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722148, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722148

Target name :

Phospho-Rad17 (S656)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to residues surrounding Ser656 of human Rad17 protein.

Uniprot id :

O75943>SwissProt: O75943 Human;SwissProt: Q6NXW6 Mouse

Host :

Rabbit

Clone number :

JE63-30

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, HeLa cells treated with 100ng/mL Calyculin A for 30 minutes.

Molecular wt :

Predicted band size: 77 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage