Histone H3 (di methyl K4) | HUABIO HA722135 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (di methyl K4)

catalog :

HA722135

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE00-98

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section, dot blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (di methyl K4) on different lysates with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722135) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling Histone H3 (di methyl K4) with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H3 (di methyl K4) with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA722135

Target name :

Histone H3 (di methyl K4)

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,ChIP,Dot Blot,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated.

Uniprot id :

P68431>SwissProt: P68431 human;SwissProt: P84243 human;SwissProt: Q16695 human;SwissProt: Q6NXT2 human;SwissProt: Q71DI3 human;SwissProt: P68433 mouse;SwissProt: P84228 mouse;SwissProt: Q6LED0 rat

Host :

Rabbit

Clone number :

JE00-98

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, HCT 116 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, NIH/3T3, PC-12, mouse colon tissue, rat colon tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500-1:2,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;Dot Blot: 1:1,000 ;IHC-P: 10,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722135_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PC-12 cells labeling Histone H3 (di methyl K4) with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722135_5.jpg

Pic legend5 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (di methyl K4) (HA722135) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722135_6.jpg

Pic legend6 :

Dot blot analysis of Histone H3 (di methyl K4) on different proteins with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (positive) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (negative) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Proteins loading: 100ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 15 seconds; ECL: K1801.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722135_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722135) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722135_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H3 (di methyl K4) antibody (HA722135) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722135) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage