Clusterin | HUABIO HA722116 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Clusterin

catalog :

HA722116

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-33

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Clusterin on different lysates with Rabbit anti-Clusterin antibody (HA722116) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: PANC-1 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: T-47D cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722116) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/2,000

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722116

Target name :

Clusterin

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Clusterin aa 400-449 / 449.

Uniprot id :

P10909>SwissProt: P10909 Human

Host :

Rabbit

Clone number :

PSH04-33

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, SK-Br-3 cell lysate, A431 cell lysate, PANC-1 cell lysate, MCF7 cell lysate, T-47D cell lysate, human lung cancer tissue, human prostate cancer tissue, human pancreas tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Nucleus, Cytoplasm, Mitochondrion membrane, cytosol, Microsome, Endoplasmic reticulum, Mitochondrion, perinuclear region, Cytoplasmic vesicle, secretory vesicle, chromaffin granule.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:8,000 ;IF-Tissue: 1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722116_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722116_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722116_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage