Human MMP-9 | HUABIO HA722106 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human MMP-9

catalog :

HA722106

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-23

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human MMP9 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722106) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP9 protein (HA210698) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722107) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 0.5%. The mean MMP9 concentration was determined to be 397.2 ng/ml in mixed serum from twenty-one volunteers.

image 3 :

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are human urine 100%. The mean MMP9 concentration was determined to be 1624.2 pg/ml in human urine.

product information

SKU :

HA722106

Target name :

Human MMP-9

Species reactivity :

Human

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MMP9 aa 20-707 (P14780).

Uniprot id :

P14780>SwissProt: P14780 Human

Host :

Rabbit

Clone number :

PSH04-23

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human MMP9 protein (HA210698).

Molecular wt :

Predicted band size: 78.5 kDa

Subcellular location :

Secreted, extracellular space, extracellular matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-24] to Human MMP9 (Detector) (HA722107) and recombinant standard Human MMP9 protein (HA210698). The reference range value is 20.6-5000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722106_4.png

Pic legend4 :

U-2 OS cells were stimulated with 200nM TPA or vehicle control in McCoy's 5a+10%FBS medium and incubated for 48 hours.The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution.Undiluted samples are TPA stimulated U-2 OS cell extract 2.5% and unstimulated U-2 OS cell extract 2.5%. The mean MMP9 concentration was determined to be 44.3 ng/ml in TPA stimulated U-2 OS cell extract and undetectable in the unstimulated U-2 OS control.

more info or order :

HUABIO product webpage