ACO1 | HUABIO HA722071 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ACO1

catalog :

HA722071

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE63-98

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ACO1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of HeLa cells labeling ACO1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722071, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA722071

Target name :

ACO1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ACO1 aa 1-50 / 889.

Uniprot id :

P21399>SwissProt: P21399 Human;SwissProt: P28271 Mouse;SwissProt: Q63270 Rat

Host :

Rabbit

Clone number :

JE63-98

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, HeLa.

Molecular wt :

Predicted band size: 98 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage