product summary
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company name :
HUABIO
product type :
antibody
product name :
Sortilin
catalog :
HA722070
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE60-00
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (HA722070) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (40 µg/Lane)
Lane 3: Human kidney tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin antibody (HA722070) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722070) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA722070
Target name :
Sortilin
Species reactivity :
Human
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Sortilin aa 601-800 / 831.
Uniprot id :
Q99523>SwissProt: Q99523 Human
Host :
Rabbit
Clone number :
JE60-00
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SK-MEL-28 cell lysate, human lung tissue lysate, human kidney tissue lysate, human liver tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, human brain tissue.
Molecular wt :
Predicted band size: 92 kDa
Subcellular location :
Golgi apparatus, Golgi stack membrane, Endosome membrane, Endoplasmic reticulum membrane, Nucleus membrane, Cell membrane, Lysosome membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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