Peroxiredoxin 5 | HUABIO HA722061 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Peroxiredoxin 5

catalog :

HA722061

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-90

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Lane 4: Mouse liver tissue lysate (15 µg/Lane) Lane 5: Mouse kidney tissue lysate (15 µg/Lane) Lane 6: Rat liver tissue lysate (15 µg/Lane) Lane 7: Rat kidney tissue lysate (15 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722061) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: MCF7 cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722061) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/20,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 1 minute 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722061) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722061

Target name :

Peroxiredoxin 5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Peroxiredoxin 5 aa 31-214 / 214.

Uniprot id :

P30044>SwissProt: P30044 Human;SwissProt: P99029 Mouse;SwissProt: Q9R063 Rat

Host :

Rabbit

Clone number :

PSH03-90

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, HepG2 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue, HeLa, F9, PC-12, C6.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Mitochondrion; Cytoplasm, Peroxisome matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:20,000-1:50,000 ;IHC-P: 1:10,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722061_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA722061_5.jpg

Pic legend5 :

Immunocytochemistry analysis of F9 cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA722061_6.jpg

Pic legend6 :

Immunocytochemistry analysis of PC-12 cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA722061_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA722061_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA722061_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA722061_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA722061_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA722061_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Peroxiredoxin 5 antibody (HA722061) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722061) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA722061_13.jpg

Pic legend13 :

Flow cytometric analysis of HeLa cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722061, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA722061_14.jpg

Pic legend14 :

Flow cytometric analysis of F9 cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722061, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA722061_15.jpg

Pic legend15 :

Flow cytometric analysis of C6 cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722061, 1μg/mL) (green) compared with Rabbit IgG Isotype Control (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img16 :

https://storage.huabio.cn/huabio/productImg/HA722061_16.jpg

Pic legend16 :

Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with HA722061 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722061 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722061 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722061 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801

Pic img17 :

https://storage.huabio.cn/huabio/productImg/HA722061_17.jpg

Pic legend17 :

Peroxiredoxin 5 was immunoprecipitated in 0.2mg PC-12 cell lysate with HA722061 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722061 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PC-12 cell lysate (input) Lane 2: HA722061 IP in PC-12 cell lysate Lane 3: Rabbit IgG instead of HA722061 in PC-12 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801

more info or order :

HUABIO product webpage