Raptor | HUABIO HA722028 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Raptor

catalog :

HA722028

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE59-39

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody (HA722028) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: K-562 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Raptor antibody (HA722028) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722028

Target name :

Raptor

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Raptor aa 1,286-1,335 / 1,335.

Uniprot id :

Q8N122>SwissProt: Q8N122 Human;SwissProt: Q8K4Q0 Mouse;Entrez Gene: 287871 Rat

Host :

Rabbit

Clone number :

JE59-39

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue.

Molecular wt :

Predicted band size: 149 kDa

Subcellular location :

Lysosome membrane, Cytoplasm, Cytoplasmic granule.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500

more info or order :

HUABIO product webpage