B7H4 | HUABIO HA722024 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

B7H4

catalog :

HA722024

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-65

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of B7H4 on different lysates with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane) Lane 2: HeLa cell lysate (negative) (30 µg/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane) Lane 4: Mouse uterus tissue lysate (30 µg/Lane) Lane 5: Rat ovary tissue lysate (15 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7H4 with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA722024

Target name :

B7H4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human B7H4 aa 101-150 / 282.

Uniprot id :

Q7Z7D3>SwissProt: Q7Z7D3 Human;SwissProt: Q7TSP5 Mouse

Host :

Rabbit

Clone number :

PSH03-65

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MDA-MB-468 cell lysate, NIH:OVCAR-3 cell lysate, mouse uterus tissue lysate, rat ovary tissue lysate, SK-Br-3.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage