p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 | HUABIO HA722019 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12

catalog :

HA722019

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-60

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: K-562 cell lysate (30 µg/Lane) Lane 4: MDA-MB-231 cell lysate (30 µg/Lane) Lane 5: COS-1 cell lysate (30 µg/Lane) Lane 6: Neuro-2a cell lysate (30 µg/Lane) Lane 7: PC-12 cell lysate (30 µg/Lane) Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA722019

Target name :

p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAPK12 aa 1-367 / 367.

Uniprot id :

Q16539>SwissProt: Q16539 Human;SwissProt: Q15759 Human;SwissProt: P53778 Human;SwissProt: P47811 Mouse;SwissProt: Q9WUI1 Mouse;SwissProt: O08911 Mouse;SwissProt: P70618 Rat;SwissProt: D4A3U7 Rat;SwissProt: Q63538 Rat

Host :

Rabbit

Clone number :

PSH03-60

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, HeLa cell lysate, K-562 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000

more info or order :

HUABIO product webpage