MELK | HUABIO HA722014 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MELK

catalog :

HA722014

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-55

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. Lane 1: Hepa1-6-si NT cell lysate Lane 2: Hepa1-6-si MELK cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (HA722014) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA722014

Target name :

MELK

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MELK aa 1-400 / 651.

Uniprot id :

Q14680>SwissProt: Q14680 Human;SwissProt: Q61846 Mouse

Host :

Rabbit

Clone number :

PSH03-55

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, MDA-MB-231 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, human breast cancer tissue, human testis tissue.

Molecular wt :

Predicted band size: 75 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:500-1:2,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA722014_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722014) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage