product summary
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company name :
HUABIO
product type :
antibody
product name :
ABCE1
catalog :
HA722$330
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE35-87
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
HA722$330
Target name :
ABCE1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ABCE1 aa 550-599 / 599.
Uniprot id :
P61221>SwissProt: P61221 Human;SwissProt: P61222 Mouse;Entrez Gene: 361390 Rat
Host :
Rabbit
Clone number :
JE35-87
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Jurkat, PC-12, mouse liver tissue, rat liver tissue.
Molecular wt :
Predicted band size: 67 kDa
Subcellular location :
Cytoplasm, Mitochondrion.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:100
;IHC-P: 1:200
;FC: 1:1,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA722$330_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ABCE1 antibody (HA722$330) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722$330) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA722$330_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ABCE1 antibody (HA722$330) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722$330) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA722$330_6.jpg
Pic legend6 :
Flow cytometric analysis of Jurkat cells labeling ABCE1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722$330, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA722$330_7.jpg
Pic legend7 :
Flow cytometric analysis of PC-12 cells labeling ABCE1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722$330, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA722$330_8.jpg
Pic legend8 :
ABCE1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722$330 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722$330 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA722$330 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722$330 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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