HIF-1 alpha | HUABIO HA721997 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HIF-1 alpha

catalog :

HA721997

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE75-33

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA721997) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate Lane 5: C2C12 cell lysate Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721997) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA721997) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate (With sonication) (15 µg/Lane) Lane 3: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate (Without sonication) (15 µg/Lane) Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721997) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (HA721997) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (HA721997) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA721997

Target name :

HIF-1 alpha

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human HIF-1 alpha aa 251-550 / 826.

Uniprot id :

Q16665>SwissProt: Q16665 Human;SwissProt: Q61221 Mouse;SwissProt: O35800 Rat

Host :

Rabbit

Clone number :

JE75-33

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate, HepG2 treated with 100μM CoCl2 for 4 hours cell lysate, C2C12 treated with 100μM CoCl2 for 4 hours cell lysate, HeLa cells treated with or without 500μM CoCl2 for 24 hours, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 93 kDa

Subcellular location :

Cytoplasm, Nucleus, Nucleus speckle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage