product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
PRMT7
catalog :
HA721996
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE64-78
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: HEK-293 cell lysate (15 µg/Lane)
Lane 5: 293T cell lysate (15 µg/Lane)
Lane 6: MCF7 cell lysate (15 µg/Lane)
Lane 7: NIH/3T3 cell lysate (15 µg/Lane)
Lane 8: Mouse testis tissue lysate (30 µg/Lane)
Lane 9: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721996) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721996
Target name :
PRMT7
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human PRMT7 aa 1-50 / 692.
Uniprot id :
Q9NVM4>SwissProt: Q9NVM4 Human;SwissProt: Q922X9 Mouse;SwissProt: Q5U4E8 Rat
Host :
Rabbit
Clone number :
JE64-78
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HEK-293 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue.
Molecular wt :
Predicted band size: 78 kDa
Subcellular location :
Cytoplasm, cytosol, Nucleus
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:1,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721996_4.jpg
Pic legend4 :
PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with HA721996 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721996 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse testis tissue lysate (input)
Lane 2: HA721996 IP in mouse testis tissue lysate
Lane 3: Rabbit IgG instead of HA721996 in mouse testis tissue lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 11 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments