Flotillin-2 | HUABIO HA721986 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Flotillin-2

catalog :

HA721986

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-41

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Flotillin-2 on different lysates with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: COS-1 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse lung tissue lysate (30 µg/Lane) Lane 7: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: Lane 1-7 (left): 6 seconds; Lane 1-7 (right): 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721986) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Flotillin-2 on different lysates with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Flotillin-2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721986) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling Flotillin-2 with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721986

Target name :

Flotillin-2

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Flotillin-2 aa 1-428 / 428.

Uniprot id :

Q14254>SwissProt: Q14254 Human;SwissProt: Q60634 Mouse;SwissProt: Q9Z2S9 Rat

Host :

Rabbit

Clone number :

PSH03-41

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A431 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, HeLa, 293T cell lysate, A549 cell lysate, MCF7 cell lysate, human lung tissue lysate, mouse skin tissue lysate, rat skin tissue lysate, NIH/3T3, PC-12, human breast cancer tissue, human liver cancer tissue, mouse lung tissue, rat lung tissue.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Cell membrane, Membrane, caveola, Endosome, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IHC-P: 1:500-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721986_4.jpg

Pic legend4 :

Western blot analysis of Flotillin-2 on different lysates with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/2,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: 293T cell lysate (30 µg/Lane) Lane 3: A549 cell lysate (30 µg/Lane) Lane 4: A431 cell lysate (30 µg/Lane) Lane 5: MCF7 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: NIH/3T3 cell lysate (30 µg/Lane) Lane 8: PC-12 cell lysate (30 µg/Lane) Lane 9: Human lung tissue lysate (30 µg/Lane) Lane 10: Mouse lung tissue lysate (30 µg/Lane) Lane 11: Mouse skin tissue lysate (hot lysis) (30 µg/Lane) Lane 12: Rat skin tissue lysate (30 µg/Lane) Lane 13: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721986) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721986_5.jpg

Pic legend5 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Flotillin-2 with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721986_6.jpg

Pic legend6 :

Immunocytochemistry analysis of PC-12 cells labeling Flotillin-2 with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721986_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721986) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721986_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721986) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA721986_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721986) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA721986_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Flotillin-2 antibody (HA721986) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721986) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA721986_11.jpg

Pic legend11 :

Flow cytometric analysis of HeLa cells labeling Flotillin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721986, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA721986_12.jpg

Pic legend12 :

Flow cytometric analysis of NIH/3T3 cells labeling Flotillin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721986, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA721986_13.jpg

Pic legend13 :

Flow cytometric analysis of PC-12 cells labeling Flotillin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721986, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage