product summary
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company name :
HUABIO
product type :
antibody
product name :
3mC
catalog :
HA721957
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH03-21
application :
dot blot
more info or order :
image
image 1 :
Immunocytochemistry analysis of U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour labeling 3mC with Rabbit anti-3mC antibody (HA721957) at 1/100 dilution.
Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCl in TBST for 30 minutes at room temperature. Permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-3mC antibody (HA721957) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
image 2 :
Dot blot analysis of 3mC on different proteins with Rabbit anti-3mC antibody (HA721957) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.
Lane 1: Uridine-BSA (negative)
Lane 2: Cytidine-BSA (negative)
Lane 3: 5-Methylcytosine-BSA (negative)
Lane 4: 3-Methylcytosine-BSA (positive)
Lane 5: 5-Hydroxymethylcytidine-BSA (negative)
Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng;
Blocking and dilution buffer: 5% NFDM/TBST;
Exposure time: 1 minute.
product information
SKU :
HA721957
Target name :
3mC
Species reactivity :
Species independent
Applications :
IF-Cell,Dot Blot
Conjugate :
Non-conjugated
Immunogen :
3-mc-OVA
Uniprot id :
>
Host :
Rabbit
Clone number :
PSH03-21
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour.
Concentration :
1 mg/mL.
Recommended dilutions :
IF-Cell: 1:100
;Dot Blot: 1:2,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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