Adipose Triglyceride Lipase | HUABIO HA721951 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Adipose Triglyceride Lipase

catalog :

HA721951

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE37-49

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: HEK-293 cell lysate (30 µg/Lane) Lane 4: A431 cell lysate (30 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/1,000 dilution. Lane 1: A431 cell lysate (30 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse white adipose tissue lysate (40 µg/Lane) Lane 4: Mouse brown adipose tissue lysate (40 µg/Lane) Lane 5: Rat heart tissue lysate (40 µg/Lane) Lane 6: Rat brown adipose tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721951

Target name :

Adipose Triglyceride Lipase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Adipose Triglyceride Lipase aa $330-450 / 504.

Uniprot id :

Q96AD5>SwissProt: Q96AD5 Human;SwissProt: Q8BJ56 Mouse;SwissProt: P0C548 Rat

Host :

Rabbit

Clone number :

JE37-49

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SiHa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A431 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, Mouse liver tissue lysate, Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, mouse brown adipose tissue, rat breast tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Lipid droplet, Cell membrane, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500-1:2,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721951_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721951_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721951, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage