HLA Class II DRB1 | HUABIO HA721927 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HLA Class II DRB1

catalog :

HA721927

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE60-43

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HLA Class II DRB1 on different lysates with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HDLM-2 cell lysate Lane 5: A375 cell lysate Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721927) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721927

Target name :

HLA Class II DRB1

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HLA Class II DRB1 aa 151-200 / 266.

Uniprot id :

P01911>SwissProt: P01911 Human;SwissProt: P01912 Human;SwissProt: P04229 Human;SwissProt: P20039 Human;SwissProt: Q29974 Human;SwissProt: Q30134 Human;SwissProt: Q30167 Human;SwissProt: Q5Y7A7 Human;SwissProt: Q95IE3 Human;SwissProt: Q9GIY3 Human

Host :

Rabbit

Clone number :

JE60-43

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HDLM-2 cell lysate, A375 cell lysate, human tonsil tissue, human skin tissue, human diffuse large B-cell lymphoma tissue.

Molecular wt :

Predicted band size: 30 kDa

Subcellular location :

Cell membrane. Endoplasmic reticulum.

Concentration :

1 mg/ml.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:100

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721927_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721927_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721927_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage