product summary
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company name :
HUABIO
product type :
antibody
product name :
Glycophorin A
catalog :
HA721920
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE32-24
reactivity :
human
application :
immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Glycophorin A antibody (HA721920) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-Glycophorin A antibody (HA721920) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Glycophorin A antibody (HA721920) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721920
Target name :
Glycophorin A
Species reactivity :
Human
Applications :
IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Glycophorin A aa 115-150 / 150.
Uniprot id :
P02724>SwissProt: P02724 Human
Host :
Rabbit
Clone number :
JE32-24
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human bone marrow tissue, human hodgkin lymphoma tissue, human kidney tissue, human placenta tissue, human spleen tissue.
Molecular wt :
Predicted band size: 16 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/ml
Recommended dilutions :
IHC-P: 1:1,000
;IF-Tissue: 1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721920_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Glycophorin A antibody (HA721920) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721920_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Glycophorin A antibody (HA721920) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721920_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded human hodgkin lymphoma tissue labeling Glycophorin A with Rabbit anti-Glycophorin A antibody (HA721920) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721920, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721920_7.jpg
Pic legend7 :
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Glycophorin A with Rabbit anti-Glycophorin A antibody (HA721920) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721920, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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