product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
ABHD5
catalog :
HA721903
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE60-62
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of ABHD5 on different lysates with Rabbit anti-ABHD5 antibody (HA721903) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 43 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721903) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of NIH/3T3 cells labeling ABHD5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721903, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA721903
Target name :
ABHD5
Species reactivity :
Mouse,Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ABHD5 aa 151-200 / 349.
Uniprot id :
Q8WTS1>SwissProt: Q8WTS1 Human;SwissProt: Q9DBL9 Mouse
Host :
Rabbit
Clone number :
JE60-62
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
NIH/3T3 cell lysate, mouse pancreas tissue lysate, mouse kidney tissue lysate, NIH/3T3.
Molecular wt :
Predicted band size: 39 kDa
Subcellular location :
Cytoplasm, Lipid droplet.
Concentration :
1 mg/ml
Recommended dilutions :
WB: 1:1,000
;FC: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments