ZHX2 | HUABIO HA721886 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ZHX2

catalog :

HA721886

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE63-11

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (HA721886) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: COS-1 cell lysate Lane 3: HeLa cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: AGS cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721886) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA721886

Target name :

ZHX2

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ZHX2 aa 251-500 / 837.

Uniprot id :

Q9Y6X8>SwissProt: Q9Y6X8 Human;SwissProt: Q8C0C0 Mouse;SwissProt: Q80VX4 Rat

Host :

Rabbit

Clone number :

JE63-11

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Neuro-2a cell lysate, COS-1 cell lysate, HeLa cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, MCF7.

Molecular wt :

Predicted band size: 92 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage