Ras | HUABIO HA721883 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ras

catalog :

HA721883

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE44-13

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (HA721883) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: A375 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Rat spleen tissue lysate (40 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721883) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (HA721883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (HA721883) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of MCF7 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721883, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721883

Target name :

Ras

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Ras aa 1-50 / 189.

Uniprot id :

P01111>SwissProt: P01111 Human;SwissProt: P01112 Human;SwissProt: P01116 Human;SwissProt: P08556 Mouse;SwissProt: P32883 Mouse;SwissProt: Q61411 Mouse;SwissProt: P08644 Rat;SwissProt: P20171 Rat;SwissProt: Q04970 Rat

Host :

Rabbit

Clone number :

JE44-13

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, A431 cell lysate, A375 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, MCF7.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Cell membrane, Golgi apparatus membrane.

Concentration :

1 mg/ml

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000

more info or order :

HUABIO product webpage