product summary
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company name :
HUABIO
product type :
antibody
product name :
GALT
catalog :
HA721876
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH02-88
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: K-562 cell lysate (15 µg/Lane)
Lane 5: THP-1 cell lysate (15 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: Lane 1-5 (left): 1 minute 34 seconds; ECL: K1801;
Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GALT KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: SiHa cell lysate (20 µg/Lane)
Lane 8: SH-SY5Y cell lysate (20 µg/Lane)
Lane 9: Mouse liver tissue lysate (40 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 43 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721876
Target name :
GALT
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human GALT aa 1-379 / 379.
Uniprot id :
P07902>SwissProt: P07902 Human;SwissProt: Q03249 Mouse
Host :
Rabbit
Clone number :
PSH02-88
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, THP-1 cell lysate, Jurkat cell lysate, SiHa cell lysate, SH-SY5Y cell lysate, mouse liver tissue lysate.
Molecular wt :
Predicted band size: 43 kDa
Subcellular location :
Cytosol, Golgi apparatus, cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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