xCT/SLC7A11 | HUABIO HA721868 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

xCT/SLC7A11

catalog :

HA721868

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE31-93

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of xCT/SLC7A11 on different lysates with Rabbit anti-xCT/SLC7A11 antibody (HA721868) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of xCT/SLC7A11 on PC-12 cell lysates with Rabbit anti-xCT/SLC7A11 antibody (HA721868) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

xCT/SLC7A11 was immunoprecipitated in 0.2mg HeLa cell lysate with HA721868 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721868 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA721868 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA721868 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801

product information

SKU :

HA721868

Target name :

xCT/SLC7A11

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human xCT aa 1-50 / 501.

Uniprot id :

Q9UPY5>SwissProt: Q9UPY5 Human;SwissProt: Q9WTR6 Mouse;Entrez Gene: 310392 Rat

Host :

Rabbit

Clone number :

JE31-93

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, PC-12 cell lysates.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage