Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) | HUABIO HA721864 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197)

catalog :

HA721864

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE45-17

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) on different lysates with Rabbit anti-Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) antibody (HA721864) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721864) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721864

Target name :

Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197)

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C-gamma.

Uniprot id :

P17612>SwissProt: P17612 Human;SwissProt: P22694 Human;SwissProt: P22612 Human;SwissProt: P05132 Mouse;SwissProt: P27791 Rat

Host :

Rabbit

Clone number :

JE45-17

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cytoplasm, Cell membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage