PML Protein | HUABIO HA721846 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PML Protein

catalog :

HA721846

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-68

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (HA721846) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: A431 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721846) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (HA721846) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PML Protein KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 15 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721846) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of MDA-MB-231 cells labeling PML Protein with Rabbit anti-PML Protein antibody (HA721846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PML Protein antibody (HA721846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721846

Target name :

PML Protein

Species reactivity :

Human,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PML Protein aa 1-600 / 882.

Uniprot id :

P29590>SwissProt: P29590 Human;Entrez Gene: 315713 Rat

Host :

Rabbit

Clone number :

PSH02-68

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, K-562 cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, MDA-MB-231, human brain tissue, human breast carcinoma tissue, human breast tissue, human placenta tissue, human testis tissue, rat breast tissue.

Molecular wt :

Predicted band size: 98 kDa

Subcellular location :

Nucleus, Cytoplasm, PML body, nucleolus, Endoplasmic reticulum membrane, Early endosome membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721846_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721846_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721846_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721846_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721846_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA721846_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-PML Protein antibody (HA721846) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721846) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage