Histone H3 (acetyl K23) | HUABIO HA721840 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (acetyl K23)

catalog :

HA721840

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE46-39

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (acetyl K23) on different lysates with Rabbit anti-Histone H3 (acetyl K23) antibody (HA721840) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721840) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with or without 500 ng/mL TSA for 4 hours labeling Histone H3 (acetyl K23) with Rabbit anti-Histone H3 (acetyl K23) antibody (HA721840) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K23) antibody (HA721840) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721840

Target name :

Histone H3 (acetyl K23)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to residues surrounding acetyl-Lys23 of human histone H3 protein.

Uniprot id :

P68431>SwissProt: P68431 Human;SwissProt: P68433 Mouse

Host :

Rabbit

Clone number :

JE46-39

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 500 ng/mL TSA for 4 hours, HeLa treated with 500 ng/mL TSA for 4 hours cell lysate, NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus. Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage