Galectin 9 | HUABIO HA721821 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Galectin 9

catalog :

HA721821

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-44

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721821) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: PC-3 cell lysate (low expression) Lane 3: PANC-1 cell lysate (low expression) Lane 4: HL-60 cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of THP-1 cells labeling Galectin 9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721821, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721821

Target name :

Galectin 9

Species reactivity :

Human,Mouse

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Galectin 9 aa 51-100 / 355.

Uniprot id :

O00182>SwissProt: O00182 Human;SwissProt: O08573 Mouse

Host :

Rabbit

Clone number :

PSH02-44

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, PANC-1 cell lysate, HL-60 cell lysate, A549 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, THP-1.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cytoplasm, Nucleus, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage