MAG | HUABIO HA721818 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAG

catalog :

HA721818

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-41

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1: 500 (MAG, HA721818, red); 1:500 (Iba1, HA601376, green) Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

product information

SKU :

HA721818

Target name :

MAG

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IF-Tissue,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAG aa 1-536 / 626.

Uniprot id :

P20916>SwissProt: P20916 Human;SwissProt: P20917 Mouse;SwissProt: P07722 Rat

Host :

Rabbit

Clone number :

PSH02-41

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain(no heat) tissue lysate, mouse cerebellum tissue lysate, rat brain(no heat) tissue lysate, rat cerebellum tissue lysate, human brain tissue, human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, mouse hippocampus tissue, rat brain tissue, rat cerebellum tissue, rat hippocampus tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Cell membrane, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:2,000-1:5,000 ;IF-Tissue: 1:200-1:1,000 ;IP: 1-2μg/sample ;IHC-Fr: 1:500-1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721818_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721818_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721818_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721818_7.jpg

Pic legend7 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:200

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721818_8.jpg

Pic legend8 :

Application: IF-tissue Species: Rat Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:200

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA721818_9.jpg

Pic legend9 :

Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane) Lane 3: Rat cerebellum tissue lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane) Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721818) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA721818_10.jpg

Pic legend10 :

Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (negative) (20 µg/Lane) Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721818) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA721818_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA721818_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA721818_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded human liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA721818_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA721818_15.jpg

Pic legend15 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img16 :

https://storage.huabio.cn/huabio/productImg/HA721818_16.jpg

Pic legend16 :

Immunohistochemical analysis of paraffin-embedded mouse liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img17 :

https://storage.huabio.cn/huabio/productImg/HA721818_17.jpg

Pic legend17 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img18 :

https://storage.huabio.cn/huabio/productImg/HA721818_18.jpg

Pic legend18 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img19 :

https://storage.huabio.cn/huabio/productImg/HA721818_19.jpg

Pic legend19 :

Immunohistochemical analysis of paraffin-embedded rat liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img20 :

https://storage.huabio.cn/huabio/productImg/HA721818_20.jpg

Pic legend20 :

MAG was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA721818 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721818 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA721818 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA721818 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 32 seconds; ECL: K1801

more info or order :

HUABIO product webpage