MTH1 | HUABIO HA721817 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MTH1

catalog :

HA721817

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-40

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lane 4: bEnd.3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: F9 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Mouse thymus tissue lysate Lane 12: Rat thymus tissue lysate Lane 13: Mouse testis tissue lysate Lane 14: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721817

Target name :

MTH1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MTH1 aa 1-156 / 156 (P36639).

Uniprot id :

P36639>SwissProt: P36639 Human;SwissProt: P53368 Mouse;SwissProt: P53369 Rat

Host :

Rabbit

Clone number :

PSH02-40

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, SW480 cell lysate, THP-1 cell lysate, SK-OV-3 cell lysate, U-2 OS cell lysate, human breast cancer tissue, NIH/3T3 cell lysate, C2C12 cell lysate, bEnd.3 cell lysate, RAW264.7 cell lysate, F9 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, human stomach cancer tissue, human thymus tissue, rat thymus tissue, Jurkat.

Molecular wt :

Predicted band size: 18 kDa

Subcellular location :

Cytoplasm, cytosol,Mitochondrion matrix,Nucleus,Mitochondrion matrix

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200~1:1,000 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721817_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721817_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721817_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721817_7.jpg

Pic legend7 :

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. Lane 1: HEK-293-si NT cell lysate Lane 2: HEK-293-si MTH1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721817_8.jpg

Pic legend8 :

Flow cytometric analysis of Jurkat cells labeling MTH1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721817,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage