product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-Tuberin (S939)
catalog :
HA721762
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE54-06
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :

Western blot analysis of Phospho-Tuberin (S939) on different lysates with Rabbit anti-Phospho-Tuberin (S939) antibody (HA721762) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate
Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour, then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 201 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721762) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721762
Target name :
Phospho-Tuberin (S939)
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phosphopeptide corresponding to residues around Ser939 of human tuberin.
Uniprot id :
P49815>SwissProt: P49815 Human;SwissProt: Q61037 Mouse
Host :
Rabbit
Clone number :
JE54-06
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate.
Molecular wt :
Predicted band size: 201 kDa
Subcellular location :
Cytoplasm, Membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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