product summary
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company name :
HUABIO
product type :
antibody
product name :
RNF114
catalog :
HA721757
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-99
reactivity :
human
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA721757 image 1
Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA721757 image 2
Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution. Lane 1: 293T-si NT cell lysate Lane 2: 293T-si RNF114 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721757
Target name :
RNF114
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human RNF114 aa 1-228 / 228.
Uniprot id :
Q9Y508>SwissProt: Q9Y508 Human
Host :
Rabbit
Clone number :
PSH01-99
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SK-Br-3 cell lysate, Jurkat cell lysate.
Molecular wt :
Predicted band size: 26 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!