FAM134B | HUABIO HA721752 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FAM134B

catalog :

HA721752

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-94

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (30 µg/Lane) Lane 2: U-2 OS cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (30 µg/Lane) Lane 5: HepG2 cell lysate (30 µg/Lane) Lane 6: TT cell lysate (30 µg/Lane) Lane 7: Jurkat cell lysate (30 µg/Lane) Lane 8: NIH/3T3 cell lysate (30 µg/Lane) Lane 9: C2C12 cell lysate (30 µg/Lane) Lane 10: PC-12 cell lysate (30 µg/Lane) Lane 11: C6 cell lysate (30 µg/Lane) Lane 12: Mouse liver tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721752

Target name :

FAM134B

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human FAM134B aa 318-497 / 497.

Uniprot id :

Q9H6L5>SwissProt:Q9H6L5 Human;SwissProt:Q8VE91 Mouse;SwissProt:Q5FVM3 Rat

Host :

Rabbit

Clone number :

PSH01-94

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, TT cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Jurkat.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

olgi apparatus, cis-Golgi network membrane, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

more info or order :

HUABIO product webpage