PSMD7 | HUABIO HA721735 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMD7

catalog :

HA721735

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-83

reactivity :

human, mouse

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of 293 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of NIH/3T3 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of K562 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA721735

Target name :

PSMD7

Species reactivity :

Human,Mouse

Applications :

IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PSMD7 aa 1-200 / 324.

Uniprot id :

P51665>SwissProt: P51665 Human;SwissProt: P26516 Mouse

Host :

Rabbit

Clone number :

PSH01-83

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293, NIH/3T3, K562, Hela.

Molecular wt :

Predicted band size: 37 kDa

Subcellular location :

Cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721735_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Hela cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage