Aldolase C | HUABIO HA721734 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Aldolase C

catalog :

HA721734

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-82

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Aldolase C on different lysates with Rabbit anti-Aldolase C antibody (HA721734) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Mouse heart tissue lysate (low expression) Lane 4: Mouse pancreas tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat hippocampus tissue lysate Lane 7: Rat heart tissue lysate (low expression) Lane 8: Rat pancreas tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721734

Target name :

Aldolase C

Species reactivity :

Mouse,Rat,Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Aldolase C aa 50-100.

Uniprot id :

P09972>SwissProt: P09972 Human;SwissProt: P05063 Mouse;SwissProt: P09117 Rat

Host :

Rabbit

Clone number :

PSH01-82

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

cytoskeleton, cytosol, extracellular exosome, extracellular region

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721734_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (negative) with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage