Aldolase A/B/C | HUABIO HA721733 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Aldolase A/B/C

catalog :

HA721733

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-81

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 3 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:1,000

product information

SKU :

HA721733

Target name :

Aldolase A/B/C

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Aldolase C aa 50-100.

Uniprot id :

P09972>SwissProt: P09972 Human;SwissProt: P05063 Mouse;SwissProt: P09117 Rat

Host :

Rabbit

Clone number :

PSH01-81

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate, U-87 MG, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

cytoskeleton, cytosol, extracellular exosome, extracellular region

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:20,000 ;IHC-Fr: 1:1,000 ;IF-Tissue: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721733_4.jpg

Pic legend4 :

Western blot analysis of Aldolase A/B/C on different lysates with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/5,000 dilution. Lane 1: U-87 MG-si NT cell lysate (10 µg/Lane) Lane 2: U-87 MG-si Aldolase A/B/C cell lysate (10 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721733) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721733_5.jpg

Pic legend5 :

Western blot analysis of Aldolase A/B/C on different lysates with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C6 cell lysate Lane 4: Human brain tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse pancreas tissue lysate (negative) Lane 7: Rat brain tissue lysate Lane 8: Rat pancreas tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 19 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721733) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721733_6.jpg

Pic legend6 :

Immunocytochemistry analysis of U-87 MG cells labeling Aldolase A/B/C with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721733_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721733) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721733_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase A/B/C antibody (HA721733) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721733) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage