KDM3A / JHDM2A | HUABIO HA721729 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KDM3A / JHDM2A

catalog :

HA721729

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-77

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: Hela cell lysate Lane 3: MCF7 cell lysate Lane 4: Ramos cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 147 kDa Observed band size: 150 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721729

Target name :

KDM3A / JHDM2A

Species reactivity :

Human,Mouse

Applications :

WB,IHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human KDM3A aa 1-400 / 1,321.

Uniprot id :

Q9Y4C1>SwissProt: Q9Y4C1 Human;SwissProt: Q6PCM1 Mouse

Host :

Rabbit

Clone number :

PSH01-77

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT116 cell lysate, Hela cell lysate, MCF7 cell lysate, Ramos cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human testis tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 147 kDa

Subcellular location :

Cytoplasm, Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC: 1:200-1:1,000

more info or order :

HUABIO product webpage