product summary
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company name :
HUABIO
product type :
antibody
product name :
IKK beta
catalog :
HA721715
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE32-81
reactivity :
human
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA721715 image 1
Western blot analysis of IKK beta on different lysates with Rabbit anti-IKK beta antibody (HA721715) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: Jurkat cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA721715 image 2
Western blot analysis of IKK beta on different lysates with Rabbit anti-IKK beta antibody (HA721715) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IKK beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721715) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
HUABIO HA721715 image 3
Flow cytometric analysis of THP-1 cells labeling IKK beta. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721715, 1/50) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA721715
Target name :
IKK beta
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human IKK beta aa 1-50 / 756.
Uniprot id :
O14920>SwissProt: O14920 Human
Host :
Rabbit
Clone number :
JE32-81
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Jurkat cell lysate, HeLa cell lysate, THP-1.
Molecular wt :
Predicted band size: 87 kDa
Subcellular location :
Cytoplasm, Nucleus, Membrane raft.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;FC: 1:50
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!