product summary
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company name :
HUABIO
product type :
antibody
product name :
KPNB1
catalog :
HA721702
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-59
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of KPNB1 on different lysates with Rabbit anti-KPNB1 antibody (HA721702) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: K-562 cell lysate (20 µg/Lane)
Lane 7: Caco-2 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: C6 cell lysate (20 µg/Lane)
Lane 11: Mouse spleen tissue lysate (40 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat spleen tissue lysate (40 µg/Lane)
Lane 14: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721702) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
image 2 :
KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with HA721702 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721702 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit IgG instead of HA721702 in HeLa cell lysate
Lane 3: HA721702 IP in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
product information
SKU :
HA721702
Target name :
KPNB1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human KPNB1 aa 1-550.
Uniprot id :
Q14974>SwissProt: Q14974 Human;SwissProt: P70168 Mouse;SwissProt: P52296 Rat
Host :
Rabbit
Clone number :
PSH01-59
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate, K-562 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse spleen tissue lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat testis tissue lysate.
Molecular wt :
Predicted band size: 97.2 kDa
Subcellular location :
Cytoplasm. Nucleus envelope.
Concentration :
1 mg/ml
Recommended dilutions :
WB: 1:1,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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