PIP5K1C | HUABIO HA721681 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PIP5K1C

catalog :

HA721681

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-43

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: SiHa cell lysate Lane 6: A549 cell lysate Lane 7: LoVo cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 87/90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PIP5K1C cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 87 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721681

Target name :

PIP5K1C

Species reactivity :

Human

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PIP5K1C aa 31-470 / 668.

Uniprot id :

O60331>SwissProt: O60331 Human

Host :

Rabbit

Clone number :

PSH01-43

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, 293T cell lysate, A431 cell lysate, SiHa cell lysate, A549 cell lysate, LoVo cell lysate.

Molecular wt :

Predicted band size: 73 kDa

Subcellular location :

Cell membrane, Endomembrane system, Cytoplasm, Cell junction, focal adhesion, adherens junction, Cell projection, ruffle membrane, phagocytic cup, uropodium; Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage